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pIK51
(Plasmid #262057)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 262057 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pDMM001
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ctc-mNG
  • Species
    B. subtilis
  • Mutation
    ctc ORF with C-terminal linker fused to mNeonGreen
  • Promoter Pveg

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.11.27.690947 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIK51 was a gift from Gene-Wei Li (Addgene plasmid # 262057 ; http://n2t.net/addgene:262057 ; RRID:Addgene_262057)

  • For your References section:

    Long-range mRNA folding shapes expression and sequence of bacterial genes. Gill MS, Kim IA, Xue JR, Thappeta Y, Taggart JC, Li GW. Mol Cell. 2026 Sep 17;86(18):3789-3802.e11. doi: 10.1016/j.molcel.2026.08.014. Epub 2026 Sep 8. 10.1016/j.molcel.2026.08.014 PubMed 42710496
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