Barcode_not_in_hairpin_background_vector
(Plasmid
#262061)
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Purpose(Empty Backbone) B. subtilis cloning vector
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Depositing Lab
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Depositing OrganizationMassachusetts Institute of Technology
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 262061 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepJCT117
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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MutationEmpty vector for Superfolder gfp 3UTR unstructured MPRA library
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Also encodes tetR.
Please visit https://doi.org/10.1101/2025.11.27.690947 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Barcode_not_in_hairpin_background_vector was a gift from Gene-Wei Li (Addgene plasmid # 262061 ; http://n2t.net/addgene:262061 ; RRID:Addgene_262061) -
For your References section:
Long-range mRNA folding shapes expression and sequence of bacterial genes. Gill MS, Kim IA, Xue JR, Thappeta Y, Taggart JC, Li GW. Mol Cell. 2026 Sep 17;86(18):3789-3802.e11. doi: 10.1016/j.molcel.2026.08.014. Epub 2026 Sep 8. 10.1016/j.molcel.2026.08.014 PubMed 42710496