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pRRO1_CMV-TOM20::TAF15N::PABPC4-delta522-bGH
(Plasmid #262119)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 262119 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pcDNA3.1/Zeo(+)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5015
  • Total vector size (bp) 7427
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TOM20::TAF15N::HA::linker::PABPC4∆C
  • Alt name
    Recordanelle-V1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2484
  • Mutation
    TAF15N with Q118H mutation, PABPC4 C-terminus deleted (522-631)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA-epitope-tag

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer cgcaaatgggcggtaggcgtg
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please consult the Supplemental Document for description of protein sequences for insert and linkers.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRRO1_CMV-TOM20::TAF15N::PABPC4-delta522-bGH was a gift from Randall Platt (Addgene plasmid # 262119 ; http://n2t.net/addgene:262119 ; RRID:Addgene_262119)

  • For your References section:

    Recordanelles for transcriptome-wide single-cell RNA recording in human cells. Reinkemeier CD, Manero-Carranza M, Farouni R, Filice F, Klotz A, Schneider-Heieck K, Andermann TL, Platt RJ. Nat Biotechnol. 2026 Oct 21. doi: 10.1038/s41587-026-03327-6. 10.1038/s41587-026-03327-6 PubMed 42907534
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