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pGEX - UHRF1
(Plasmid #262157)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 262157 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pGEX-4T-1
  • Backbone manufacturer
    GE Healthcare
  • Backbone size w/o insert (bp) 4900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    UHRF1
  • Species
    H. sapiens (human)
  • Mutation
    includes aa 108-287 only
  • GenBank ID
    NP_001041666.1
  • Entrez Gene
    UHRF1 (a.k.a. ICBP90, Np95, RNF106, TDRD22, hNP95, hUHRF1, huNp95)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

UniProt ID: Q96T88

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX - UHRF1 was a gift from Mark Bedford (Addgene plasmid # 262157 ; http://n2t.net/addgene:262157 ; RRID:Addgene_262157)

  • For your References section:

    Histone peptide microarray screen of chromo and Tudor domains defines new histone lysine methylation interactions. Shanle EK, Shinsky SA, Bridgers JB, Bae N, Sagum C, Krajewski K, Rothbart SB, Bedford MT, Strahl BD. Epigenetics Chromatin. 2017 Mar 14;10:12. doi: 10.1186/s13072-017-0117-5. eCollection 2017. 10.1186/s13072-017-0117-5 PubMed 28293301
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