pGEX - TDRD6-6
(Plasmid
#262168)
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PurposeProtein expression of the Tudor domain 6 from TDRD6 in bacteria
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Depositing Lab
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Depositing OrganizationUniversity of Texas MD Anderson Cancer Center
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 262168 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepGEX-4T-1
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Backbone manufacturerGE Healthcare
- Backbone size w/o insert (bp) 4900
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTDRD6
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SpeciesH. sapiens (human)
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Mutationincludes aa 1295-1432 only
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GenBank IDNP_001161831.1
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Entrez GeneTDRD6 (a.k.a. TDR2, CT41.2, FLJ43850, NY-CO-45, bA446F17.4, DKFZp781I1148)
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
UniProt ID: O60522
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX - TDRD6-6 was a gift from Mark Bedford (Addgene plasmid # 262168 ; http://n2t.net/addgene:262168 ; RRID:Addgene_262168) -
For your References section:
Histone peptide microarray screen of chromo and Tudor domains defines new histone lysine methylation interactions. Shanle EK, Shinsky SA, Bridgers JB, Bae N, Sagum C, Krajewski K, Rothbart SB, Bedford MT, Strahl BD. Epigenetics Chromatin. 2017 Mar 14;10:12. doi: 10.1186/s13072-017-0117-5. eCollection 2017. 10.1186/s13072-017-0117-5 PubMed 28293301