pSHHygMALDHB
(Plasmid
#262473)
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PurposeMaltose-inducible expression of 4,4′-dihydroxybenzil-dependent Cre
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Depositing Lab
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Depositing OrganizationQueensland University of Technology
Located in Australia -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 262473 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepUC19
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Vector typeYeast Expression
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Selectable markersHygromycin, URA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePMAL32-hER*-Cre-TCYC1
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SpeciesS. cerevisiae (budding yeast)
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Entrez GeneMAL32 (a.k.a. YBR299W)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Cre is still leaky. Cre has a T327N mutation.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSHHygMALDHB was a gift from Bingyin Peng (Addgene plasmid # 262473 ; http://n2t.net/addgene:262473 ; RRID:Addgene_262473)