pToxEXPERG20
(Plasmid
#262474)
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PurposeRelE-expressing ToxAmp vector including the GAL promoter-driven ERG20 expression cassette
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Depositing Lab
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Depositing OrganizationQueensland University of Technology
Located in Australia -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 262474 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepUC19
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Vector typeYeast Expression
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHO(-955,-789)-KlURA3-PGAL1-ERG20-TRPL3t-PCUP1-RelE(RPL28intron)-TTPI1-HO(-721,-264)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
ERG20 has G167D, E308D, and I318V mutations.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pToxEXPERG20 was a gift from Bingyin Peng (Addgene plasmid # 262474 ; http://n2t.net/addgene:262474 ; RRID:Addgene_262474)