4xNRE-min-LUC
(Plasmid
#262478)
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PurposeLuciferase reporter for measuring NLP-dependent nitrate-responsive transcription in a protoplast system.
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Depositing Lab
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Depositing OrganizationMassachusetts General Hospital
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 262478 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepHBT
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Vector typePlant Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nameFirefly luciferase
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Insert Size (bp)1655
Gene/Insert 2
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Gene/Insert nameRenilla luciferase
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Insert Size (bp)936
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
4xNRE-min-LUC was a gift from Jen Sheen (Addgene plasmid # 262478 ; http://n2t.net/addgene:262478 ; RRID:Addgene_262478) -
For your References section:
NIN-like protein 7 transcription factor is a plant nitrate sensor. Liu KH, Liu M, Lin Z, Wang ZF, Chen B, Liu C, Guo A, Konishi M, Yanagisawa S, Wagner G, Sheen J. Science. 2022 Sep 23;377(6613):1419-1425. doi: 10.1126/science.add1104. Epub 2022 Sep 22. 10.1126/science.add1104 PubMed 36137053