pAG36-NPL3-3xHA
(Plasmid
#262529)
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PurposeExpresses NPL3 with C-terminal 3xHA tag under its native promoter
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Depositing Lab
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Depositing OrganizationUniversity of Ottawa
Located in Canada -
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 262529 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepAG36
- Backbone size w/o insert (bp) 6123
- Total vector size (bp) 8169
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Vector typeYeast Expression
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Selectable markersURA3 ; Nourseothricin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNpl3
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)1373
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MutationSequence has been codon optimized
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Entrez GeneNPL3 (a.k.a. YDR432W, MTR13, MTS1, NAB1, NOP3)
- Promoter Native
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Tag
/ Fusion Protein
- 3xHA (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer None
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAG36-NPL3-3xHA was a gift from Michael Downey (Addgene plasmid # 262529 ; http://n2t.net/addgene:262529 ; RRID:Addgene_262529)
