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pBAD-HiCaRI HA
(Plasmid #262557)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 262557 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pBAD
  • Backbone size w/o insert (bp) 4041
  • Total vector size (bp) 5286
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HiCaRI HA
  • Insert Size (bp)
    1245
  • Promoter araBAD
  • Tag / Fusion Protein
    • His-tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer CTGTTTCTCCATACCCGTTTTTTGGGC
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://www.biorxiv.org/content/10.64898/2026.03.06.710044v1 for the bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBAD-HiCaRI HA was a gift from Robert Campbell (Addgene plasmid # 262557 ; http://n2t.net/addgene:262557 ; RRID:Addgene_262557)

  • For your References section:

    A StayGold-based calcium ion indicator. Miyazaki I, Tsao KK, Terai T, Takahashi-Yamashiro K, Campbell RE. bioRxiv 2026.03.06.710044 10.64898/2026.03.06.710044
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