Skip to main content
Addgene
Open Menu

Xlone-MYCN_mcherryNLS
(Plasmid #262607)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 262607 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart
DNA 262607-D50 50 μg of DNA in Tris buffer $495
Add to Cart
DNA 262607-D100 100 μg of DNA in Tris buffer $585
Add to Cart

Backbone

  • Vector backbone
    XLone backbone, no drug-resistance gene
  • Backbone manufacturer
    Addgene #140032
  • Backbone size w/o insert (bp) 6591
  • Total vector size (bp) 7247
  • Modifications to backbone
    Modified from XLone-eGFP NLS (Addgene #140032): the EF1α-EGFP cassette was replaced with EF1α-mCherry-NLS, and the MYCN coding sequence (amplified from PB-TRE3G-MYCN, Addgene #104542) was inserted via SpeI/KpnI digestion, replacing EGFP to enable doxycycline-inducible MYCN expression.
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MYCN
  • Alt name
    N-Myc
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1431
  • GenBank ID
    4613
  • Entrez Gene
    MYCN (a.k.a. FGLDS1, MODED, MPAPA, MYCNsORF, MYCNsPEP, N-myc, NMYC, ODED, bHLHe37)
  • Promoter TRE3GS

Cloning Information

  • Cloning method Other
  • 5′ sequencing primer cctaccctcgtaaaggtaccatgccgagctgct
  • 3′ sequencing primer gaggtggtctactagtctagcaagtccgagcgtgttca
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    PB-TRE3G-MYCN, Addgene #104542

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.64898/2026.01.27.701690 for bioRxiv preprint.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Xlone-MYCN_mcherryNLS was a gift from Margareta Wilhelm (Addgene plasmid # 262607 ; http://n2t.net/addgene:262607 ; RRID:Addgene_262607)
Related items: