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pcDNA3.1(+) ZKSCAN1 Scarless-Split eGFP
(Plasmid #262621)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 262621 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Backbone manufacturer
    Life Technologies
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    eGFP
  • Species
    Synthetic
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer TAATACGACTCACTATAGGG
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1(+) ZKSCAN1 Scarless-Split eGFP was a gift from Jeremy Wilusz (Addgene plasmid # 262621 ; http://n2t.net/addgene:262621 ; RRID:Addgene_262621)

  • For your References section:

    Engineering circular RNA expression systems to minimize contaminating linear RNA byproducts. Fields CJ, Fujiwara R, Wright BW, Stringer BW, Conn SJ, Wilusz JE. bioRxiv [Preprint]. 2026 Sep 11:2026.09.07.749908. doi: 10.64898/2026.09.07.749908. 10.64898/2026.09.07.749908 PubMed 42818330
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