pcDNA3.1(+) circRNA Mini-NFASC Exons 26-27
(Plasmid
#262650)
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PurposeExpresses scarred circNFASC(26,27) circRNA in mammailian cells using mini ZKSCAN1 introns
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Depositing Lab
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Depositing OrganizationBaylor College of Medicine
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 262650 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepcDNA3.1(+)
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Backbone manufacturerLife Technologies
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNFASC
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SpeciesH. sapiens (human)
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Insert Size (bp)23114
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer TAATACGACTCACTATAGGG
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1(+) circRNA Mini-NFASC Exons 26-27 was a gift from Jeremy Wilusz (Addgene plasmid # 262650 ; http://n2t.net/addgene:262650 ; RRID:Addgene_262650)