Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26272||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7560
Vector typeMammalian Expression, Lentiviral, RNAi, Cre/Lox
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namep21 shRNA
SpeciesM. musculus (mouse)
Insert Size (bp)55
- Cloning method Restriction Enzyme
- 5′ cloning site HpaI (destroyed during cloning)
- 3′ cloning site XhoI (unknown if destroyed)
- 5′ sequencing primer TGCAGGGGAAAGAATAGTAGAC (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
The shRNA is directed against the mouse p21 sequence 5'- gcagattggtcttctgcaa -3'.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:PSICORhp-mp21(v2) was a gift from Rudolf Jaenisch (Addgene plasmid # 26272 ; http://n2t.net/addgene:26272 ; RRID:Addgene_26272)
For your References section:Direct cell reprogramming is a stochastic process amenable to acceleration. Hanna J, Saha K, Pando B, van Zon J, Lengner CJ, Creyghton MP, van Oudenaarden A, Jaenisch R. Nature. 2009 Dec 3. 462(7273):595-601. 10.1038/nature08592 PubMed 19898493