pMBP-AsCas12a (K607R)
(Plasmid
#262786)
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PurposeExpresses 10xHis-MBP fused to AsCas12a with K607R
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Depositing Lab
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Depositing OrganizationUniversity of California, Riverside
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 262786 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepMBP
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Vector typeBacterial Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAsCas12a (K607R)
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MutationK607R
- Promoter T7
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Tag
/ Fusion Protein
- 10xHis-MBP (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer GATGAAGCCCTGAAAGACGCGCAG
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMBP-AsCas12a (K607R) was a gift from Juhong Chen (Addgene plasmid # 262786 ; http://n2t.net/addgene:262786 ; RRID:Addgene_262786) -
For your References section:
CRISPR in MOF Formulation with Enhanced Stability, Activity, and Altered PAM Specificity for Broad-Spectrum Diagnosis of Bacterial Sepsis. Pal T, Liu Z, Nair MG, Chen J. Adv Sci (Weinh). 2026 Feb;13(8):e13439. doi: 10.1002/advs.202513439. Epub 2025 Nov 23. 10.1002/advs.202513439 PubMed 41276946