pKEK3479
(Plasmid
#262792)
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Purpose(Empty Backbone) EUREKA-AE destination vector for construction of vectors for allelic exchange
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Depositing Lab
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Depositing OrganizationUniversity of Texas at San Antonio
Located in the United States of America -
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 262792 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepUC-SpecR-oriT
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Vector typeAllelic Exchange
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Contains BsaI-eforCP from Echinopara forskaliana.
Universal primer overhangs for assembly: Forward 5'- ggctacGGTCTCgCCCG-3' Reverse 5'- ggctacGGTCTCgGCAG-3'
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKEK3479 was a gift from Karl Klose (Addgene plasmid # 262792 ; http://n2t.net/addgene:262792 ; RRID:Addgene_262792)
