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pEGFPC1-DesmoplakinHead TagRFPT Periplakin Tail
(Plasmid #262819)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 262819 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 262819-D50 50 μg of DNA in Tris buffer $495
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DNA 262819-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pEGFPC1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Desmoplakin, Periplakin
  • Alt name
    DSP (a.k.a. DCWHKTA, DP)
  • Alt name
    Periplakin;PPL
  • Species
    H. sapiens (human)
  • Entrez Gene
    DSP (a.k.a. DCWHKTA, DP)
  • Entrez Gene
    PPL
  • Promoter CMV

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    made by Bageshri Nanavati

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

To synthesize pC1 Linker construct, a gblock was synthesized containing 176 AA from the N-terminus of DP and 111 AA from the C-terminus of periplakin. The gblock was cloned into p-C1 vector by restriction digestion with Age1 and BamH1. Linker construct was subsequently cloned into a pLVX-IRES-Puro lentiviral backbone by restriction digestion with Age1 and Hpa1.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFPC1-DesmoplakinHead TagRFPT Periplakin Tail was a gift from Alpha Yap (Addgene plasmid # 262819 ; http://n2t.net/addgene:262819 ; RRID:Addgene_262819)

  • For your References section:

    Desmosome-anchored intermediate filaments facilitate tension-sensitive RhoA signaling for epithelial homeostasis. Nanavati BN, Noordstra I, Verma S, Duszyc K, Green KJ, Yap AS. bioRxiv [Preprint]. 2023 Feb 23:2023.02.23.529786. doi: 10.1101/2023.02.23.529786. 10.1101/2023.02.23.529786 PubMed 36865131
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