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Tet-on-HA-PUMA-PGK-mCherry-CAAX
(Plasmid #262820)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 262820 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pLL5.0
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PUMA
  • Alt name
    BBC3 (a.k.a. JFY-1, JFY1, PUMA)
  • Species
    H. sapiens (human)
  • Entrez Gene
    BBC3 (a.k.a. JFY-1, JFY1, PUMA)
  • Promoter LTR viral promoter
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    made by Bageshri Nanavati

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Tet-on-HA-PUMA-PGK-mCherry-CAAX was a gift from Alpha Yap (Addgene plasmid # 262820 ; http://n2t.net/addgene:262820 ; RRID:Addgene_262820)

  • For your References section:

    Desmosome-anchored intermediate filaments facilitate tension-sensitive RhoA signaling for epithelial homeostasis. Nanavati BN, Noordstra I, Verma S, Duszyc K, Green KJ, Yap AS. bioRxiv [Preprint]. 2023 Feb 23:2023.02.23.529786. doi: 10.1101/2023.02.23.529786. 10.1101/2023.02.23.529786 PubMed 36865131
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