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PX459_APCKO
(Plasmid #262823)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 262823 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pSpCas9 (BB)-2A-Puro (PX459) vector
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sgRNAs targeting APC exon 1
  • Alt name
    APC (a.k.a. BTPS2, DESMD, DP2, DP2.5, DP3, GS, PPP1R46)
  • gRNA/shRNA sequence
    50-GCAAGTTGAGGCACTGAAGA-30
  • Species
    H. sapiens (human)
  • Entrez Gene
    APC (a.k.a. BTPS2, DESMD, DP2, DP2.5, DP3, GS, PPP1R46)
  • Promoter CMV

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    made by Wan Jun Gang

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PX459_APCKO was a gift from Alpha Yap (Addgene plasmid # 262823 ; http://n2t.net/addgene:262823 ; RRID:Addgene_262823)

  • For your References section:

    A truncation mutant of adenomatous polyposis coli impairs apical cell extrusion through elevated epithelial tissue tension. Gan WJ, Giri R, Begun J, Abud HE, Hardeman EC, Gunning PW, Yap AS, Noordstra I. Cytoskeleton (Hoboken). 2025 Jul;82(7):444-455. doi: 10.1002/cm.21893. Epub 2024 Jul 10. 10.1002/cm.21893 PubMed 38984538
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