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pCas-LbCas12a-Q888P-N889P_pCB482-p15A
(Plasmid #262849)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 262849 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pCB482
  • Backbone manufacturer
    Beckwith Lab (pACYC184-derived, p15A origin)
  • Backbone size w/o insert (bp) 4067
  • Total vector size (bp) 7751
  • Modifications to backbone
    LbCas12a expressed from the constitutive PJ23108 promoter
  • Vector type
    Bacterial Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Cloned and propagated in E. coli DH10B, LB at 37 C with chloramphenicol. No special propagation requirement is known; standard recA1 endA1 cloning strains are expected to be suitable.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    LbCas12a
  • Alt name
    LbCpf1
  • Alt name
    Cas12a
  • Alt name
    Cpf1
  • Species
    Lachnospiraceae bacterium ND2006
  • Insert Size (bp)
    3684
  • Mutation
    Q888P, N889P in the bridge helix

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCas-LbCas12a-Q888P-N889P_pCB482-p15A was a gift from John van der Oost (Addgene plasmid # 262849 ; http://n2t.net/addgene:262849 ; RRID:Addgene_262849)
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