pCMV6-Nrf1-EGFP
(Plasmid
#262892)
-
PurposeExpresses Nrf1 fused with EGFP under control of CMV promoter.
-
Depositing Lab
-
Depositing OrganizationRutgers, The State University of New Jersey
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 262892 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
-
Vector backbonepCMV6
-
Backbone manufacturerOriGene
- Backbone size w/o insert (bp) 4122
- Total vector size (bp) 7032
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameNrf1
-
SpeciesM. musculus (mouse)
-
Insert Size (bp)1527
-
GenBank IDNM_001164226
-
Entrez GeneNrf1 (a.k.a. D6Ertd415e)
- Promoter CMV
-
Tag
/ Fusion Protein
- EGFP (N terminal on insert)
Cloning Information
- Cloning method Golden Gate
- 5′ sequencing primer caccaaaatcaacgggactt
- (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made byModified from OriGene plasmid MR226726, pCMV6-Nrf1.
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCMV6-Nrf1-EGFP was a gift from Ronald Hart (Addgene plasmid # 262892 ; http://n2t.net/addgene:262892 ; RRID:Addgene_262892) -
For your References section:
ATM is activated by ATP depletion and modulates mitochondrial function through NRF1. Chow HM, Cheng A, Song X, Swerdel MR, Hart RP, Herrup K. J Cell Biol. 2019 Mar 4;218(3):909-928. doi: 10.1083/jcb.201806197. Epub 2019 Jan 14. 10.1083/jcb.201806197 PubMed 30642892