YIplac211-Kex2-HaloTagx2
(Plasmid
#262943)
-
PurposeInsert a C-terminal HaloTagx2 at KEX2 by popin-popout
-
Depositing Lab
-
Depositing OrganizationUniversity of Chicago
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 262943 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
-
Vector backboneYIplac211
-
Vector typeYeast Expression
-
Selectable markersURA3
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameKEX2-HaloTagx2
-
SpeciesS. cerevisiae (budding yeast), Synthetic
-
Entrez GeneKEX2 (a.k.a. YNL238W, QDS1, SRB1, VMA45)
Resource Information
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.64898/2026.05.20.726668 for the bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
YIplac211-Kex2-HaloTagx2 was a gift from Benjamin Glick (Addgene plasmid # 262943 ; http://n2t.net/addgene:262943 ; RRID:Addgene_262943) -
For your References section:
Functional assignment of Golgi-associated vesicle tethers to specific membrane recycling pathways. Krahn AH, Johnson N, Austin J 2nd, Glick BS. bioRxiv [Preprint]. 2026 May 21:2026.05.20.726668. doi: 10.64898/2026.05.20.726668. 10.64898/2026.05.20.726668 PubMed 42239406