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pGY036-BSMV_Gamma_PaqcI-ccdb-PaqcI-sgRNA scaffold_T-DNA
(Plasmid #262956)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 262956 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    BSMVγ vector (pYL157)
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This vector contains the sgRNA backbone, the ccdB gene flanked on both sites by AarI for the insertion of target sequence, and has to be used with BSMV alpha and beta genomes (Addgene # 262954 and 262955).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGY036-BSMV_Gamma_PaqcI-ccdb-PaqcI-sgRNA scaffold_T-DNA was a gift from Daniel Voytas (Addgene plasmid # 262956 ; http://n2t.net/addgene:262956 ; RRID:Addgene_262956)

  • For your References section:

    BSMV-mediated genome editing exhibits host-specific heritability: germline transmission in barley and somatic edits in Nicotiana benthamiana. Bhowmik PK, Williams JT, Polley B, Chen N, Kavuri NR, Zang W, Barakate A, Yang H, Narra MK, Beattie AD, Starker C, Voytas DF, Baysal C. BMC Plant Biol. 2026 May 12;26(1):1132. doi: 10.1186/s12870-026-08866-3. 10.1186/s12870-026-08866-3 PubMed 42115922
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