FSW-iGluSNFR
(Plasmid #262957)

• Purpose: Lentiviral plasmid expressing iGluSNFR from the human synapsin promoter.
• Depositing Lab: Ronald Hart
• Depositing Organization: Rutgers, The State University of New Jersey
  Located in the United States of America
• Publication: Hart Lab Plasmids (unpublished)

Backbone

• Vector backbone: FUGW
• Backbone manufacturer: David Baltimore (Addgene Plasmid #14883)
• Backbone size w/o insert (bp): 7910
• Total vector size (bp): 10193
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: iGluSNFR
• Species: Synthetic
• Insert Size (bp): 1824
• Promoter: hSyn

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: gggaggggaaacaggatg

Resource Information

• A portion of this plasmid was derived from a plasmid made by: David Baltimore, Lenti vector came from FUGW (Addgene #14883); Kaspar Podgorski, iGluSNFR came from pAAV.hSyn.iGluSnFR3.v857.GPI (Addgene # 178331).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry