pAAV-stuffer-P(hSyn1)-mCherry
(Plasmid
#262966)
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Purpose(Empty Backbone) An empty AAV vector designed for cloning and expression of three tandem gRNA expression units, with an hSyn1-driven mCherry reporter for monitoring transduction.
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Depositing Lab
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Depositing OrganizationThe University of Tokyo
Located in Japan -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 262966 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepAAV
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-stuffer-P(hSyn1)-mCherry was a gift from Hideki Ukai (Addgene plasmid # 262966 ; http://n2t.net/addgene:262966 ; RRID:Addgene_262966) -
For your References section:
Postsynaptic competition between calcineurin and PKA regulates mammalian sleep-wake cycles. Wang Y, Cao S, Tone D, Fujishima H, Yamada RG, Ohno RI, Shi S, Matsuzawa K, Yada S, Kaneko M, Sakamoto H, Onishi T, Ukai-Tadenuma M, Ukai H, Hanashima C, Hirose K, Kiyonari H, Sumiyama K, Ode KL, Ueda HR. Nature. 2024 Dec;636(8042):412-421. doi: 10.1038/s41586-024-08132-2. Epub 2024 Nov 6. 10.1038/s41586-024-08132-2 PubMed 39506111