pAAV-gRNA-P(hSyn)mCherry_template vector 1
(Plasmid
#262969)
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Purpose(Empty Backbone) A template vector for PCR amplification of hU6 promoter-driven gRNA expression units encoding user-defined gRNAs.
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Depositing Lab
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Depositing OrganizationThe University of Tokyo
Located in Japan -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 262969 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepAAV
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Vector typeMammalian Expression, AAV
- Promoter hU6
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-gRNA-P(hSyn)mCherry_template vector 1 was a gift from Hideki Ukai (Addgene plasmid # 262969 ; http://n2t.net/addgene:262969 ; RRID:Addgene_262969) -
For your References section:
Postsynaptic competition between calcineurin and PKA regulates mammalian sleep-wake cycles. Wang Y, Cao S, Tone D, Fujishima H, Yamada RG, Ohno RI, Shi S, Matsuzawa K, Yada S, Kaneko M, Sakamoto H, Onishi T, Ukai-Tadenuma M, Ukai H, Hanashima C, Hirose K, Kiyonari H, Sumiyama K, Ode KL, Ueda HR. Nature. 2024 Dec;636(8042):412-421. doi: 10.1038/s41586-024-08132-2. Epub 2024 Nov 6. 10.1038/s41586-024-08132-2 PubMed 39506111