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pGEM-T-easy-gRNA-hU6_template vector 2
(Plasmid #262970)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 262970 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pGEM-T-easy
  • Vector type
    Mammalian Expression, Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The pGEM-T Easy vector backbone was purchased from Promega Corporation. The gRNA-hU6 expression cassette was cloned into this vector in our laboratory.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEM-T-easy-gRNA-hU6_template vector 2 was a gift from Hideki Ukai (Addgene plasmid # 262970 ; http://n2t.net/addgene:262970 ; RRID:Addgene_262970)

  • For your References section:

    Postsynaptic competition between calcineurin and PKA regulates mammalian sleep-wake cycles. Wang Y, Cao S, Tone D, Fujishima H, Yamada RG, Ohno RI, Shi S, Matsuzawa K, Yada S, Kaneko M, Sakamoto H, Onishi T, Ukai-Tadenuma M, Ukai H, Hanashima C, Hirose K, Kiyonari H, Sumiyama K, Ode KL, Ueda HR. Nature. 2024 Dec;636(8042):412-421. doi: 10.1038/s41586-024-08132-2. Epub 2024 Nov 6. 10.1038/s41586-024-08132-2 PubMed 39506111
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