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pJHA830
(Plasmid #262977)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 262977 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    RP4/RSK
  • Vector type
    Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Pir1
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cre-NanoLuc
  • Species
    Synthetic

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.11.08.687059 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJHA830 was a gift from Mark Mimee (Addgene plasmid # 262977 ; http://n2t.net/addgene:262977 ; RRID:Addgene_262977)
  • For your References section:

    Engineering butyrate-producing Lachnospiraceae to treat metabolic disease. Arnold J, McClure S, Glazier J, Ahan RE, Shakya S, Villegas D, Chen TL, Fuerte-Stone J, McGann R, Mimee M. bioRxiv [Preprint]. 2025 Nov 9:2025.11.08.687059. doi: 10.1101/2025.11.08.687059. 10.1101/2025.11.08.687059 PubMed 41279522