pJHA830
(Plasmid
#262977)
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PurposeA plasmid containing Clostridium optimized cre recombinase, the lox71 attP site and an inducible NanoLuc sequence.
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Depositing Lab
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Depositing OrganizationUniversity of Chicago
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 262977 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backboneRP4/RSK
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Vector typeCre/Lox
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Pir1
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCre-NanoLuc
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SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2025.11.08.687059 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJHA830 was a gift from Mark Mimee (Addgene plasmid # 262977 ; http://n2t.net/addgene:262977 ; RRID:Addgene_262977) -
For your References section:
Engineering butyrate-producing Lachnospiraceae to treat metabolic disease. Arnold J, McClure S, Glazier J, Ahan RE, Shakya S, Villegas D, Chen TL, Fuerte-Stone J, McGann R, Mimee M. bioRxiv [Preprint]. 2025 Nov 9:2025.11.08.687059. doi: 10.1101/2025.11.08.687059. 10.1101/2025.11.08.687059 PubMed 41279522