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pJHA1174
(Plasmid #262983)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 262983 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    ColE1
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 12.5 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NanoLuc
  • Species
    Synthetic

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.11.08.687059 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJHA1174 was a gift from Mark Mimee (Addgene plasmid # 262983 ; http://n2t.net/addgene:262983 ; RRID:Addgene_262983)

  • For your References section:

    Engineering commensal Lachnospiraceae for programmable therapeutic delivery in the mouse gut. Arnold J, McClure S, Glazier J, Shakya S, Jansen Z, Ahan RE, Villegas D, Chen TL, Fuerte-Stone J, McGann R, Mimee M. Nat Microbiol. 2026 Nov 4. doi: 10.1038/s41564-026-02527-9. 10.1038/s41564-026-02527-9 PubMed 42982882
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