pET21a-human-alpha-synuclein
(Plasmid
#263036)
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PurposeExpresses codon-optimized human alpha-synuclein
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Depositing Lab
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Depositing OrganizationDuke University
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 263036 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepET21a
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsDepositor recommends growing plasmid in BL21 DE3 competent cells.
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Copy numberUnknown
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET21a-human-alpha-synuclein was a gift from Andrew West (Addgene plasmid # 263036 ; http://n2t.net/addgene:263036 ; RRID:Addgene_263036) -
For your References section:
Mouse alpha-synuclein fibrils are structurally and functionally distinct from human fibrils associated with Lewy body diseases. Sokratian A, Zhou Y, Tatli M, Burbidge KJ, Xu E, Viverette E, Donzelli S, Duda AM, Yuan Y, Li H, Strader S, Patel N, Shiell L, Malankhanova T, Chen O, Mazzulli JR, Perera L, Stahlberg H, Borgnia M, Bartesaghi A, Lashuel HA, West AB. Sci Adv. 2024 Nov;10(44):eadq3539. doi: 10.1126/sciadv.adq3539. Epub 2024 Nov 1. 10.1126/sciadv.adq3539 PubMed 39485845