pBEL2982
(Plasmid
#263107)
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PurposeTo express mScarlet3 in mammalian cells
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Depositing Lab
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Depositing OrganizationJohns Hopkins University and School of Medicine
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 263107 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepLV
- Backbone size w/o insert (bp) 6814
- Total vector size (bp) 7504
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemScarlet3
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SpeciesSynthetic
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Insert Size (bp)690
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer cgcccgggcgtacgggatcccaccggtgcatggatagcaccgaggcagtgatcaag
- 3′ sequencing primer ctctacaaatgtggtatggctgattatgatctagttaggagccaccggagccgccggtg
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byBackbone comes from Addgene plasmid # 44386 mScarlet3 gene comes from Addgene plasmid # 189754
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBEL2982 was a gift from Damian Ekiert (Addgene plasmid # 263107 ; http://n2t.net/addgene:263107 ; RRID:Addgene_263107)