pMAL-HTTex1(Q44)-C-6XHis
(Plasmid #263186)

• Purpose: Expresses MBP-HTTex1(Q44)-C-His in bacterial cells.
• Depositing Lab: Judith Frydman
• Depositing Organization: Stanford University
  Located in the United States of America
• Publication: pCDNA HTT Variable Q-Length Expression Plasmids (unpublished)

Backbone

• Vector backbone: pMAL
• Backbone manufacturer: New England Biolabs
• Backbone size w/o insert (bp): 5453
• Total vector size (bp): 7019
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HTT Exon 1
• Alt name: HTT1a
• Species: H. sapiens (human)
• Insert Size (bp): 1566
• Mutation: CAG/Glutamine repeat length of 25.
• GenBank ID: NM_002111.8 NM_002111.8
• Entrez Gene: HTT (a.k.a. HD, IT15, LOMARS)
• Promoter: TAC Promoter
• Tags / Fusion Proteins:
  • Maltose Binding Protein (N terminal on insert)
  • 6xHis (C terminal on insert)

Cloning Information

• Cloning method: Other
• 5′ sequencing primer: GTTTAGGTGTTTTCACGAGCACTTCAC

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Poirier, M., et al., Huntingtin Spheroids and Protofibrils as Precursors in Polyglutamine Fibrilization. Journal of Biological Chemistry, 2002; 277, 41032-41037. https://doi.org/10.1074/jbc.M205809200

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry