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pKC010
(Plasmid #263188)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 263188 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pUC19
  • Backbone size w/o insert (bp) 2629
  • Total vector size (bp) 5977
  • Vector type
    Algal expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Neomycin resistance
  • Alt name
    nptII
  • Alt name
    G418
  • Species
    Auxenochlorella protothecoides x symbiontica UTEX 250-A
  • Insert Size (bp)
    1840
  • Promoter ApxsPGK1

Cloning Information

  • Cloning method Other
  • 5′ sequencing primer accaactagtCCTTCCTGTCCCACAATG
  • 3′ sequencing primer gttggagcctCTTTGCGCTCTCATCTGG
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.64898/2026.09.26.754548 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKC010 was a gift from Krishna Niyogi (Addgene plasmid # 263188 ; http://n2t.net/addgene:263188 ; RRID:Addgene_263188)

  • For your References section:

    A Natural qE- and qZ-Deficient Alga Retains Functional Plant-Like and Chlorophycean Violaxanthin De-Epoxidases. Cunningham KW, Wakao S, Hall JL, Blaby-Haas C, Ivatury R, Gleeson L, Chuang D, Kim JJ, Moseley JL, Fleming GR, Niyogi KK. bioRxiv 2026.09.26.754548 10.64898/2026.09.26.754548
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