pFP12
(Plasmid #263238)

• Purpose: Stable shuttle vector for use in N. gonorrhoeae, Haemophilus spp, and other bacteria. pFP10 derivative that allows for fusion of insert with GFP.
• Depositing Lab: Jo-Anne Dillon
• Depositing Organization: University of Ottawa
  Located in Canada
• Publication: Pagotto et al Gene. 2000 Feb 22;244(1-2):13-9. doi: 10.1016/s0378-1119(99)00557-0.

Backbone

• Vector backbone: pFP10
• Total vector size (bp): 6567
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: EGFP
• Alt name: GFP
• Species: Synthetic
• Insert Size (bp): 721
• Promoter: lac promoter

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (destroyed during cloning)
• 3′ cloning site: HindIII (destroyed during cloning)
• 5′ sequencing primer: EGFP-N
• 3′ sequencing primer: EGFP-C

Resource Information

• A portion of this plasmid was derived from a plasmid made by: plac-MCS-GFP-MCS sequence was amplified from the plasmid pEGFP (Clontech).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry