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pCY005
(Plasmid #263521)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 263521 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pGJJ001
  • Backbone size w/o insert (bp) 6305
  • Modifications to backbone
    Substituted 1 CYC promoter for a GPD promoter and added Fyn substrate, Fyn kinase, and SH2 phosphoamino acid binding domain.
  • Vector type
    Bacterial Expression, Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Fyn substrate-Fyn kinase
  • Species
    Synthetic
  • Insert Size (bp)
    864
  • Promoter CYC

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer TTACAGTTAATAAAGCGTTTGG
  • 3′ sequencing primer gtgaatgtaagcgtgac
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    SH2
  • Species
    Synthetic
  • Insert Size (bp)
    303
  • Promoter GPD

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SpeI (not destroyed)
  • 5′ sequencing primer aggtgggtcaggtggaggcggatccCGCATGCATTGGTACTTTG
  • 3′ sequencing primer gctcgaaggctttaatttgactagtttaACAGACGTTTGTCAGCCTG
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.11.07.687244 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCY005 was a gift from David Van Valen (Addgene plasmid # 263521 ; http://n2t.net/addgene:263521 ; RRID:Addgene_263521)

  • For your References section:

    Uncovering the molecular basis of kinase activity and substrate recognition with phospho-PCA. Yu C, Pao E, Van Valen D. bioRxiv [Preprint]. 2025 Nov 13:2025.11.07.687244. doi: 10.1101/2025.11.07.687244. 10.1101/2025.11.07.687244 PubMed 41278632
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