pJET-piezo2a-Ribo-Rv-1
(Plasmid
#263525)
-
PurposeAntisense riboprobe template for zebrafish piezo2a WISH.
-
Depositing Lab
-
Depositing OrganizationPurdue University
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 263525 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
-
Vector backbonepJET1.2
-
Backbone manufacturerThermo Fisher Scientific
- Backbone size w/o insert (bp) 2974
- Total vector size (bp) 4158
-
Vector typeCloning Vector
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namepiezo2a
-
Alt namePiezo2.a
-
Alt nameLOC100334952
-
SpeciesD. rerio (zebrafish)
-
Insert Size (bp)1184
-
GenBank IDXM_021470255.2
-
Entrez Genepiezo2a (a.k.a. piezo1b, piezo2a.1, piezo2a.2, si:ch1073-433p8.1)
- Promoter T7
Cloning Information
- Cloning method Other
- 5′ sequencing primer pJET1.2 Forward Sequencing Primer
- 3′ sequencing primer pJET1.2 Reverse Sequencing Primer
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.64898/2026.05.15.725496 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pJET-piezo2a-Ribo-Rv-1 was a gift from GuangJun Zhang (Addgene plasmid # 263525 ; http://n2t.net/addgene:263525 ; RRID:Addgene_263525) -
For your References section:
Piezo3 is a new member of the PIEZO ion channel family in vertebrates. Dong Z, Wang D, Wang B, New JA, Leung YF, Zhang G. Dev Biol. 2026 Oct 8:S0012-1606(26)00206-X. doi: 10.1016/j.ydbio.2026.10.001. 10.1016/j.ydbio.2026.10.001 PubMed 42849651