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AAV-CAG-DIO-mWGA-mCherry
(Plasmid #263584)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 263584 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    AAV2-CAG-DIO expression vector
  • Backbone size w/o insert (bp) 4723
  • Total vector size (bp) 7163
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    mWGA
  • Alt name
    mammalian codon-optimized wheat germ agglutinin
  • Insert Size (bp)
    528
  • Promoter CAG

Cloning Information for Gene/Insert 1

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer CAG For
  • 3′ sequencing primer WPRE Rev
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    mCherry
  • Insert Size (bp)
    711
  • Promoter CAG

Cloning Information for Gene/Insert 2

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer CAG For
  • 3′ sequencing primer WPRE Rev
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV-CAG-DIO-mWGA-mCherry was a gift from Xin Duan (Addgene plasmid # 263584 ; http://n2t.net/addgene:263584 ; RRID:Addgene_263584)

  • For your References section:

    Trans-Seq maps a selective mammalian retinotectal synapse instructed by Nephronectin. Tsai NY, Wang F, Toma K, Yin C, Takatoh J, Pai EL, Wu K, Matcham AC, Yin L, Dang EJ, Marciano DK, Rubenstein JL, Wang F, Ullian EM, Duan X. Nat Neurosci. 2022 May;25(5):659-674. doi: 10.1038/s41593-022-01068-8. Epub 2022 May 6. 10.1038/s41593-022-01068-8 PubMed 35524141
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