AAV-CAG-DIO-mYFP
(Plasmid
#263586)
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PurposeCre-dependent fluorescent reporter for cell-type-specific axon labeling
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Depositing Lab
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Depositing OrganizationUniversity of California, San Francisco (UCSF)
Located in the United States of America -
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 263586 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backboneAAV2-CAG-DIO expression vector
- Backbone size w/o insert (bp) 6046
- Total vector size (bp) 6842
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemYFP
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Alt namemembrane-targeted EYFP
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Insert Size (bp)796
- Promoter CAG
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AscI (not destroyed)
- 3′ cloning site NheI (not destroyed)
- 5′ sequencing primer CAG For
- 3′ sequencing primer WPRE Rev
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AAV-CAG-DIO-mYFP was a gift from Xin Duan (Addgene plasmid # 263586 ; http://n2t.net/addgene:263586 ; RRID:Addgene_263586)
Map uploaded by the depositor.
