pAAV.CB6-CLuc-T2A-MeCP2 R168X-GLuc
(Plasmid
#263989)
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PurposeDual-luciferase PTC reporter with in-frame UGA premature termination codon to detect nonsense suppression; AAV genome expressing wild-type CLuc and GLuc with the T2A and Mecp2 R168X sequence context
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Depositing Lab
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Depositing OrganizationUniversity of Massachusetts, Worcester
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 263989 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepAAV
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCLuc-T2A-Mcep2 R168X context-GLuc
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SpeciesC. noctiluca; G. princeps
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MutationNonsense mutation in Mecp2 R168 context
- Promoter CB6
Cloning Information
- Cloning method Gibson Cloning
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV.CB6-CLuc-T2A-MeCP2 R168X-GLuc was a gift from Dan Wang (Addgene plasmid # 263989 ; http://n2t.net/addgene:263989 ; RRID:Addgene_263989) -
For your References section:
An engineered UGA suppressor tRNA gene for disease-agnostic AAV delivery. Xu M, Liu H, Wang J, Vieira AFC, Zhou X, Liu N, Liang J, Du A, Chen X, Xing R, Yang Y, Gonzalez-Perez MP, Kumar V, Wang D. Nat Biotechnol. 2026 Jan 19. doi: 10.1038/s41587-025-02982-5. 10.1038/s41587-025-02982-5 PubMed 41555020