pCMV-mIdua W401X (TGA)-Flag
(Plasmid
#263995)
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PurposeMammalian expression plasmid for mouse Idua-FLAG with W401X (TGA) mutation
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Depositing Lab
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Depositing OrganizationUniversity of Massachusetts, Worcester
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 263995 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepCMV
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namemouse Idua W401X (TGA)-flag
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SpeciesM. musculus (mouse)
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MutationW401X nonsense mutation in Idua
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-mIdua W401X (TGA)-Flag was a gift from Dan Wang (Addgene plasmid # 263995 ; http://n2t.net/addgene:263995 ; RRID:Addgene_263995) -
For your References section:
An engineered UGA suppressor tRNA gene for disease-agnostic AAV delivery. Xu M, Liu H, Wang J, Vieira AFC, Zhou X, Liu N, Liang J, Du A, Chen X, Xing R, Yang Y, Gonzalez-Perez MP, Kumar V, Wang D. Nat Biotechnol. 2026 Jan 19. doi: 10.1038/s41587-025-02982-5. 10.1038/s41587-025-02982-5 PubMed 41555020