pBAD-Drt1b-3Q
(Plasmid
#264025)
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PurposeInducible expression of Drt1b-3Q mutant
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Depositing Lab
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Depositing OrganizationUniversity of Southern California
Located in the United States of America -
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 264025 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepBAD33.1
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Backbone manufacturerChristian Raetz, Addgene plasmid #36267
- Backbone size w/o insert (bp) 5439
- Total vector size (bp) 5900
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameDrt1b
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SpeciesKlebsiella pneumoniae NCTC9143
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Insert Size (bp)432
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MutationR69;72;85Q
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer pBAD-F
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBAD-Drt1b-3Q was a gift from Douglas Feldman (Addgene plasmid # 264025 ; http://n2t.net/addgene:264025 ; RRID:Addgene_264025)
