pTN1058-pTACxc-T2gp21
(Plasmid
#264084)
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PurposeExpresses T2 gp21 under IPTG-inducible promoter.
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Depositing Lab
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Depositing OrganizationUniversity of Colorado, Boulder
Located in the United States of America -
Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 264084 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepTACxc
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameT2 gp21
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SpeciesEscherichia phage T2
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GenBank IDBBF63181.1
- Promoter Ptac
Cloning Information
- Cloning method Gibson Cloning
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.12.17.629029 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTN1058-pTACxc-T2gp21 was a gift from Aaron Whiteley (Addgene plasmid # 264084 ; http://n2t.net/addgene:264084 ; RRID:Addgene_264084) -
For your References section:
A bacterial NLR-related protein senses distinct phage triggers through a single interface. Robbins LK, Kibby EM, Deep A, Min NK, Whalen LA, Borja Najera LD, Nagy TA, Freeborn L, Armbruster EG, Pogliano J, Corbett KD, Whiteley AT. bioRxiv 2024.12.17.629029 10.1101/2024.12.17.629029