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pLR0098-pLOCO3-bNACHT11-D2A_P3A
(Plasmid #264096)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 264096 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pLOCO3
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    The depositing lab recommends using carbenicillin for antibiotic selection.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    bNACHT11
  • Species
    Klebsiella pneumoniae
  • Mutation
    D2A; P3A
  • GenBank ID
    UFEQ01000017.1
  • Promoter Native promoter

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.12.17.629029 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLR0098-pLOCO3-bNACHT11-D2A_P3A was a gift from Aaron Whiteley (Addgene plasmid # 264096 ; http://n2t.net/addgene:264096 ; RRID:Addgene_264096)

  • For your References section:

    A bacterial NLR-related protein senses distinct phage triggers through a single interface. Robbins LK, Kibby EM, Deep A, Min NK, Whalen LA, Borja Najera LD, Nagy TA, Freeborn L, Armbruster EG, Pogliano J, Corbett KD, Whiteley AT. bioRxiv 2024.12.17.629029 10.1101/2024.12.17.629029
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