p-ECFP-N1-BirA-neurexin
(Plasmid #26413)

• Depositing Lab: Alice Ting
• Publication: Thyagarajan et al Cell. 2010 Oct 6. ():.

Backbone

• Vector backbone: p-ECFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: BirA-NRX
• Alt name: Neurexin
• Alt name: NRX 1beta
• Species: R. norvegicus (rat); E. coli BirA
• Insert Size (bp): 2307
• Mutation: Deleted A146 in the BirA gene
• Entrez Gene: Nrxn1 (a.k.a. Nrxn1b)
• Tag / Fusion Protein:
  • Myc (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: sequence
• 3′ sequencing primer: sequence

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dr. Ann Marie Craig (UBC Vancouver)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

BirA-NRX was derived from CFP-NRX1beta(SS-4) from Graf et al 2004, by replacing CFP with Myc tagged BirA within EcoR1 and SpeI sites.