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p-ECFP-N1-BirA-neurexin
(Plasmid #26413)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26413 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    p-ECFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BirA-NRX
  • Alt name
    Neurexin
  • Alt name
    NRX 1beta
  • Species
    R. norvegicus (rat); E. coli BirA
  • Insert Size (bp)
    2307
  • Mutation
    Deleted A146 in the BirA gene
  • Entrez Gene
    Nrxn1 (a.k.a. Nrxn1b)
  • Tag / Fusion Protein
    • Myc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer sequence
  • 3′ sequencing primer sequence
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Ann Marie Craig (UBC Vancouver)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

BirA-NRX was derived from CFP-NRX1beta(SS-4) from Graf et al 2004, by replacing CFP with Myc tagged BirA within EcoR1 and SpeI sites.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p-ECFP-N1-BirA-neurexin was a gift from Alice Ting (Addgene plasmid # 26413)

  • For your References section:

    Imaging Activity-Dependent Regulation of Neurexin-Neuroligin Interactions Using trans-Synaptic Enzymatic Biotinylation. Thyagarajan A, Ting AY. Cell. 2010 Oct 6. ():. 10.1016/j.cell.2010.09.025 PubMed 20933261
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