|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26423||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2880
Vector typeMammalian Expression, Adenoviral
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namePGK-b-geo, HSV-TK, CAG-Venus
SpeciesE. coli, HSV, jellyfish
Insert Size (bp)28690
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI, SwaI, SalI (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Note that Addgene was unable to obtain very much sequence for this plasmid, but our diagnostic digest (see Reviews) suggests that it is correct. We encourage users to do their own diagnostics.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pHDAdVenus-geo-TK was a gift from Kohnosuke Mitani (Addgene plasmid # 26423 ; http://n2t.net/addgene:26423 ; RRID:Addgene_26423)
For your References section:Highly efficient transient gene expression and gene targeting in primate embryonic stem cells with helper-dependent adenoviral vectors. Suzuki K, Mitsui K, Aizawa E, Hasegawa K, Kawase E, Yamagishi T, Shimizu Y, Suemori H, Nakatsuji N, Mitani K. Proc Natl Acad Sci U S A. 2008 Sep 16. 105(37):13781-6. 10.1073/pnas.0806976105 PubMed 18768795