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pcDNA3-Flag mTOR wt Notes

Addgene Diagnostic Digest for 26603

This gel depicts the NEB 1kb ladder and a digestion of the plasmid 26603 with the NotI-HF enzyme. The expected pattern of bands was observed.

Addgene Diagnostic Digest

This gel depicts the NEB 1kb DNA ladder and a digestion of plasmid 26603 with EcoRI-HF.

Expected banding pattern:

EcoRI-HF: 3 bands at 9, 3 and 1 kb.

Addgene Diagnostic Digest

This gel depicts the NEB 1kb ladder and a digestion of the plasmid pcDNA3-Flag mTOR wt with the following enzymes: HpaI and PvuI-HF.