Skip to main content
Addgene
Open Menu

pFLAG-Fbw7α-3'UTR
(Plasmid #26650)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 26650 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart
DNA 26650-D50 50 μg of DNA in Tris buffer $495
Add to Cart
DNA 26650-D100 100 μg of DNA in Tris buffer $585
Add to Cart

Backbone

  • Vector backbone
    p3XFLAG-Myc-CMV
  • Backbone manufacturer
    Sigma
  • Backbone size w/o insert (bp) 4800
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Fbw7α
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5467
  • Tag / Fusion Protein
    • 3XFLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer hGH-pA-R
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

3'UTR of FBXW7 immediately follows the stop codon at the end of the FBXW7 reading frame.

C-terminal myc tag present in vector will not be expressed as fusion protein due to FBXW7 stop codon.

Addgene NGS results identified a D705E variant on the C-terminus of FBXW7, which also lacks the final two residues (MK).

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFLAG-Fbw7α-3'UTR was a gift from Alex Minella (Addgene plasmid # 26650 ; http://n2t.net/addgene:26650 ; RRID:Addgene_26650)

  • For your References section:

    MicroRNA-223 regulates cyclin E activity by modulating expression of F-box and WD-40 domain protein 7. Xu Y, Sengupta T, Kukreja L, Minella AC. J Biol Chem. 2010 Nov 5;285(45):34439-46. doi: 10.1074/jbc.M110.152306. Epub 2010 Sep 7. 10.1074/jbc.M110.152306 PubMed 20826802
Related items:
Commonly requested with: