VZV ORF47
(Plasmid #26698)

• Depositing Lab: Robert Kalejta
• Publication: Kuny et al PLoS Pathog. 2010 . 6(9):.

Backbone

• Vector backbone: pCGN
• Backbone size w/o insert (bp): 5000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ORF47
• Species: Varicella Zoster Virus (HHV-3)
• Mutation: A 3B3 epitope tag (DQRQYGDVFKGD) from the VZV gB protein is inserted at amino acid #50
• Entrez Gene: ORF47
• Tag / Fusion Protein:
  • HA (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: Bglob-pA-R

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

ORF47 accession no.: AF314218.1