pCS2+ centrin-RFP
(Plasmid #26753)

• Depositing Lab: Christopher Kintner
• Publication: Park et al Nat Genet. 2008 Jul . 40(7):871-9.

Backbone

• Vector backbone: pCS2+
• Backbone size w/o insert (bp): 4097
• Vector type: reporter for basal body

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Centrin 4
• Alt name: cetn4
• Species: X. laevis (frog)
• GenBank ID: NP_001090133
• Entrez Gene: cetn4 (a.k.a. MGC130946)
• Tag / Fusion Protein:
  • monomeric RFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: XbaI (unknown if destroyed)
• 5′ sequencing primer: SP6

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry