pMSCV FHA-ATG12
(Plasmid #27049)

• Depositing Lab: Jayanta Debnath
• Publication: Radoshevich et al Cell. 2010 Aug 20. 142(4):590-600.

Backbone

• Vector backbone: pMSCV
• Backbone size w/o insert (bp): 6300
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: autophagy-related 12 (yeast)
• Alt name: Apg12l
• Alt name: Atg12l
• Alt name: Atg12
• Species: M. musculus (mouse)
• Insert Size (bp): 530
• GenBank ID: NM_026217
• Entrez Gene: Atg12 (a.k.a. 4931423H11Rik, A330058M13Rik, Apg12l, Atg12l)
• Tags / Fusion Proteins:
  • flag (N terminal on insert)
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: NotI (destroyed during cloning)
• 5′ sequencing primer: n/a

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry